First European interlaboratory comparison of tetracycline and age determination with red fox teeth following oral rabies vaccination programs.
Identifieur interne : 000260 ( Main/Exploration ); précédent : 000259; suivant : 000261First European interlaboratory comparison of tetracycline and age determination with red fox teeth following oral rabies vaccination programs.
Auteurs : Emmanuelle Robardet [France] ; Jean-Michel Demerson ; Sabrina Andrieu ; Florence CliquetSource :
- Journal of wildlife diseases [ 1943-3700 ] ; 2012.
English descriptors
- KwdEn :
- Administration, Oral, Age Determination by Teeth (methods), Age Determination by Teeth (veterinary), Age Factors, Animals, Biomarkers (analysis), Europe, Foxes, Laboratories (standards), Mandible, Rabies Vaccines (administration & dosage), Reproducibility of Results, Sensitivity and Specificity, Tetracycline (analysis).
- MESH :
- chemical , administration & dosage : Rabies Vaccines.
- chemical , analysis : Biomarkers, Tetracycline.
- geographic : Europe.
- methods : Age Determination by Teeth.
- standards : Laboratories.
- veterinary : Age Determination by Teeth.
- Administration, Oral, Age Factors, Animals, Foxes, Mandible, Reproducibility of Results, Sensitivity and Specificity.
Abstract
The first European interlaboratory comparison of tetracycline and age determination with red fox (Vulpes vulpes) tooth samples was organized by the European Union Reference Laboratory for rabies. Performance and procedures implemented by member states were compared. These techniques are widely used to monitor bait uptake in European oral rabies vaccination campaigns. A panel of five red fox half-mandibles comprising one weak positive juvenile sample, two positive adult samples, one negative juvenile sample, and one negative adult sample were sent, along with a technical questionnaire, to 12 laboratories participating on a voluntary basis. The results of only three laboratories (25%) were 100% correct. False-negative results were more frequently seen in weak positive juvenile samples (58%) but were infrequent in positive adult samples (4%), probably due to differences in the ease of reading the two groups of teeth. Four laboratories (44%) had correct results for age determination on all samples. Ages were incorrectly identified in both adult and juvenile samples, with 11 and 17% of discordant results, respectively. Analysis of the technical questionnaires in parallel with test results suggested that all laboratories cutting mandible sections between the canine and first premolar obtained false results. All the laboratories using longitudinal rather than transverse sections and those not using a mounting medium also produced false results. Section thickness appeared to affect the results; no mistakes were found in laboratories using sections <150 μm thick. Factors having a potential impact on the success of laboratories were discussed, and recommendations proposed. Such interlaboratory trials underline the importance of using standardized procedures for biomarker detection in oral rabies vaccination campaigns. Several changes can be made to improve analysis quality and increase the comparability of bait uptake frequencies among member states.
DOI: 10.7589/2011-07-205
PubMed: 23060487
Affiliations:
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Le document en format XML
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Determination by Teeth</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Administration, Oral</term><term>Age Factors</term><term>Animals</term><term>Foxes</term><term>Mandible</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The first European interlaboratory comparison of tetracycline and age determination with red fox (Vulpes vulpes) tooth samples was organized by the European Union Reference Laboratory for rabies. Performance and procedures implemented by member states were compared. These techniques are widely used to monitor bait uptake in European oral rabies vaccination campaigns. A panel of five red fox half-mandibles comprising one weak positive juvenile sample, two positive adult samples, one negative juvenile sample, and one negative adult sample were sent, along with a technical questionnaire, to 12 laboratories participating on a voluntary basis. The results of only three laboratories (25%) were 100% correct. False-negative results were more frequently seen in weak positive juvenile samples (58%) but were infrequent in positive adult samples (4%), probably due to differences in the ease of reading the two groups of teeth. Four laboratories (44%) had correct results for age determination on all samples. Ages were incorrectly identified in both adult and juvenile samples, with 11 and 17% of discordant results, respectively. Analysis of the technical questionnaires in parallel with test results suggested that all laboratories cutting mandible sections between the canine and first premolar obtained false results. All the laboratories using longitudinal rather than transverse sections and those not using a mounting medium also produced false results. Section thickness appeared to affect the results; no mistakes were found in laboratories using sections <150 μm thick. Factors having a potential impact on the success of laboratories were discussed, and recommendations proposed. Such interlaboratory trials underline the importance of using standardized procedures for biomarker detection in oral rabies vaccination campaigns. Several changes can be made to improve analysis quality and increase the comparability of bait uptake frequencies among member states.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Malzéville</li></settlement></list><tree><noCountry><name sortKey="Andrieu, Sabrina" sort="Andrieu, Sabrina" uniqKey="Andrieu S" first="Sabrina" last="Andrieu">Sabrina Andrieu</name><name sortKey="Cliquet, Florence" sort="Cliquet, Florence" uniqKey="Cliquet F" first="Florence" last="Cliquet">Florence Cliquet</name><name sortKey="Demerson, Jean Michel" sort="Demerson, Jean Michel" uniqKey="Demerson J" first="Jean-Michel" last="Demerson">Jean-Michel Demerson</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Robardet, Emmanuelle" sort="Robardet, Emmanuelle" uniqKey="Robardet E" first="Emmanuelle" last="Robardet">Emmanuelle Robardet</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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